This is a single issue of Methods, a companion journal to Methods in Enzymology, focusing on rapidly developing techniques. This single issue is dedicated to Macromolecular Crystallization and begins with an Introduction to Protein Crystallization by Alexander McPherson.
The issue features the following additional thirteen articles:
•Protein crystallization and phase diagrams, Neer Asherie.
•Growth and disorder of macromolecular crystals: insights from atomic force microscopy and x-ray diffraction studies, Alexander J. Malkin and Robert E. Thorne.
•Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process, Kim D. Collins.
•Effects of naturally occurring osmolytes on protein stability and solubility; issues important in protein crystallization, D.W. Bolen.
•Practical aspects of using the microbatch method in screening conditions for protein crystallization, Allan D’Arcy, Aengus Mac Sweeney, and Alexander Haber.
•Automated systems for protein crystallization, Joel Bard, Kimberly Ercolani, Kristine Svenson, Andrea Olland, and Will Somers.
•Lipidic cubic phases as matrices for membrane protein crystallization, Peter Nollert.
•The use of recombinant and molecular engineering in protein crystallization, Zygmunt Derewenda.
•A pedestrian guide to membrane protein crystallization, Michael Wiener.
•Crystallization data mining in structural genomics: using positive and negative results to optimize protein crystallization screens, Rebecca Page and Raymond C. Stevens.
•Predictive models for protein crystallization, Bernhard Rupp and Junwen Wang.
•Crystallization of RNA and RNA-protein complexes, Ailong Ke and Jennifer A. Doudna.
•Macromolecular cryocrystallography – methods for cooling and mounting protein crystals are cryogenic temperatures, J.W. Pflugrath
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