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蛋白晶体研究工具和试剂
℡ 4000-520-616
℡ 4000-520-616
Hampton research/MembFac • Crystal Screen Lite • MembFac HT/MembFac /10 ml, tube format/HR2-114
产品编号:HR2-114
市  场 价:¥5060.00
场      地:美国(厂家直采)
产品分类: 其它>耗材>其他>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$253.00
品      牌: Hampton research
公      司:HAMPTON RESEARCH CORP.
公司分类:
Hampton research/MembFac • Crystal Screen Lite • MembFac HT/MembFac /10 ml, tube format/HR2-114
商品介绍
Applications

Features

Description

MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins.

MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2 Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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品牌介绍

美国Hampton Research公司是蛋白质晶体研究方面的专家,是一个从事蛋白质晶体研究研制的专业公司,为全球科研人员提供晶体研究工具和试剂,完整的晶体培养体系;同时提供晶体设计和合成方面的服务。其提供的试剂长期以来在该领域居于领先地位,为广大研究人员提供了极大的方便,促进了蛋白质晶体的研究,为世界范围内的蛋白质晶体研究人员所信赖。

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