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蛋白晶体研究工具和试剂
℡ 4000-520-616
℡ 4000-520-616
Hampton research/Nucleic Acid Mini Screen (NAM)/(+/-)-2-Methyl-2,4-pentanediol/35% v/v - 200 ml/HR2-863
产品编号:HR2-863
市  场 价:¥900.00
场      地:美国(厂家直采)
产品分类: 其它>耗材>其他>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$45.00
品      牌: Hampton research
公      司:HAMPTON RESEARCH CORP.
公司分类:
Hampton research/Nucleic Acid Mini Screen (NAM)/(+/-)-2-Methyl-2,4-pentanediol/35% v/v - 200 ml/HR2-863
商品介绍
Applications

Features

Description

The Nucleic Acid Mini Screen is an efficient screen formulated to assist in the determination of preliminary crystallization conditions of nucleic acid fragments.1 The formulation is based upon the publication, “A Highly Efficient 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments” where the preliminary crystallization conditions of 35 nucleic acids were determined utilizing this formulation.1

Samples include DNA-Drug complexes, C-Tetrad and G-Quartet Motifs, RNA oligomers, and others. By using 1 to 4 mM oligonucleotide stock concentration, the screen requires less than 100 µl of sample. The screen is typically performed at 4°C although room temperature incubations can also be performed. To evaluate the effect of equilibration kinetics as well as initial and final sample concentrations, the screens are typically performed using the hanging or sitting drop vapor diffusion method with two drops on a slide (1 µl + 2 µl and 2 µl + 2 µl), side by side. The 24 well format of the mini screen provides for a fast setup and uses small amounts of sample. This makes it possible to cost-effectively screen many sequences as well as variations of a particular sequence. The composition of the Nucleic Acid Mini Screen allows one to apply the formulation to other nucleic acids such as deoxy- and ribozymes, pseudoknots, and tRNAs.

Each Nucleic Acid Mini Screen kit (HR2-118) contains 24 unique reagents, 1.0 ml each. All solutions are formulated using ultra-pure Type 1 water and are sterile filtered. Dehydrants is available separately as 200 ml of 35% v/v (+/-)-2-Methyl-2,4-pentanediol in deionized water(catalog number HR2-863). Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) and sterile filtered into sterile containers.

Note: Crystallization kit (HR2-118) and Dehydrant/reservoir solution (HR2-863) are sold separately. Both items are needed to perform the screen.

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品牌介绍

美国Hampton Research公司是蛋白质晶体研究方面的专家,是一个从事蛋白质晶体研究研制的专业公司,为全球科研人员提供晶体研究工具和试剂,完整的晶体培养体系;同时提供晶体设计和合成方面的服务。其提供的试剂长期以来在该领域居于领先地位,为广大研究人员提供了极大的方便,促进了蛋白质晶体的研究,为世界范围内的蛋白质晶体研究人员所信赖。

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